web analytics

Injectable Calcium Phosphate Amorphous Nanoparticle Pastes for Orthopedic Applications

Interests: Musculoskeletal
Published:
Lead Inventor: Juan de Pablo
Print or Save to PDF

SUMMARY

  • Calcium phosphates have chemical similarity to bones and teeth, and as such, these compounds are highly desired for orthopedic and dental implant applications. However, current formulations have limited bioavailability and compressive strength, which limits the clinical utility of these materials.
  • The inventors developed a method to synthesize polymer stabilized calcium phosphate nanoparticles. The synthesis protocol yields amorphous nanoparticles, which have improved bioavailability and compressive strength over alternatively synthesized nanoparticles.
  • The product is a polymer stabilized calcium phosphate nanoparticle formulation deliverable as an injectable paste. The size and morphology of the nanoparticles can be fine-tuned by attenuating the synthesis protocol.
  • Proof-of-concept experiments demonstrated the bioavailability of the calcium phosphate nanoparticles: a variety of seeded mesenchymal stem cell lines (MSCs) showed high viability and higher expression of osteogenic differentiation markers than when MSCs were cultured without the nanoparticles. 

 

FIGURE

MSCs were either transfected with GFP (negative control) or with the osteogenic differentiation inducing factor BMP-9 and cultured in the presence or absence of the calcium phosphate nanoparticle paste. The expression of various osteogenic differentiation markers was then measured. The nanoparticle paste acts synergistically with BMP-9 to induce marker expression in certain cases (a), and can also act independently of BMP-9 for other markers (b, c).

 

 

ADVANTAGES

ADVANTAGES

  • Improved bioavailability
  • Improved compressive strength
  • Synthesized in situ
  • Adjustable nanoparticle size and morphology

 

APPLICATIONS

  • Orthopedic implants/bone grafting
  • Dental implants
  • Regenerative medicine
  • 3-D printing

 

PUBLICATIONS

 

 

  • US: 16/084,864