Method for the in Vitro Generation of Trabecular Bone

SUMMARY

• Trabecular (Cancellous) bone is a porous bone found in the spine and hip and is desirable for bone grafting because of its osteogenic properties. Given the difficulty of harvesting bone from the hip and spine, a method for the in vitro generation of trabecular bone is highly desired but all prior attempts have failed.
• The investigators discovered that when the Clnc3 gene is knocked out in osteoblasts, the resulting cells produce bone in the trabecular pattern with a mineral density high enough for grafting applications.
• The product is a method for generating trabecular bone in a bioreactor by first knocking out the Clcn3 gene in mesenchymal stem cells, and then culturing them in the presence of osteoblast differentiation media. Trabecular bone is harvested from the bioreactor at the end of the culture period.
• The investigators cultured a murine Clcn3-/- mesenchymal stem cell line for 2 weeks in osteoblast differentiation media. They used silver staining at the 2-week timepoint to show the formation of trabecular bone in the bioreactor with the cultured knockout cell line, but not in the wild type cell line.

FIGURE

ADVANTAGES

ADVANTAGES

• Trabecular bone production and grafting without painful harvesting procedure
• First and only reported in vitro trabecular bone generation method

APPLICATIONS

• Regenerative medicine
• Surgical implants

PUBLICATIONS

• Larrouture, QC; et al. Chloride-hydrogen antiporters ClC-3 and ClC-5 drive osteoblast mineralization and regulate fine-structure bone patterning in vitro. Physiol Rep. 2015 Nov; 3(11).

• Blair, HC; et al. Osteoblast differentiation and bone matrix formation in vivo and in vitro. Tissue Eng Part B Rev. 2017 Jun; 23(3): 268-280.

• US:15/760,452