SUMMARY Enhanced spatial RNA labeling accuracy and efficiency through bioorthogonal acylating agents that enable precise proximity-dependent RNA modification and imaging, improving cellular RNA mapping for both research and clinical molecular diagnostics The Unmet Need: Current RNA proximity-labeling methods lack specificity and efficiency for precise subcellular RNA mapping Traditional RNA labeling approaches… Read More
SUMMARY Tumor Interstitial Fluid Medium (TIFM) is a pancreatic tumor-mimetic cell culture formulation, paired with matched murine PDAC cell lines adapted in either TIFM or RPMI-1640, that is designed to model nutrient conditions in the pancreatic tumor microenvironment more faithfully than standard media. The Unmet Need: Standard cell culture media… Read More
SUMMARY The Unmet Need: Methods for efficient, reversible integration and excision of large DNA Genome engineering is a rapidly advancing field with immense potential for synthetic biology and gene therapy. The ability to precisely modify DNA sequences within living cells is crucial… Read More
SUMMARY Engineered reverse transcriptases (RTs) to profile epigenetic modifications have numerous advantages over traditional antibody enrichment methods including: single base pair resolution, lower sample quantities needed, and lower noise and background interference. Problematically, current RT engineering strategies are cumbersome, low throughput, and cannot be versatilely applied to multiple types of… Read More