Sequencing

PUM-seq: Chemical Labeling Method For Single-Nucleotide Mapping of Pseudouridine Across Species

SUMMARY Improved single-nucleotide-resolution profiling of pseudouridine across diverse RNA types is offered by a chemical-labeling and mutation-based sequencing method that detects and quantifies this modification under mild conditions The Unmet Need: RNA pseudouridine mapping remains limited by inefficient and biased detection methods Pseudouridine (Ψ) is a widespread RNA modification whose… Read More

DMN-seq: DEMETER-Assisted 5-Methylcytosine Nicking Sequencing Profiling From Sub-Nanogram Cell-Free DNA

SUMMARY More sensitive methylation profiling from scarce clinical samples is supported by a sequencing method that uses 5mC-specific glycosylase activity to detect methylated sites at single-base resolution and enrich hypomethylated DNA The Unmet Need: Existing methylation methods struggle to profile hypomethylated DNA in scarce clinical samples Bisulfite and related conversion… Read More

New Reversible Uridine Modification Chemistry For Labeling and Functionalizing Native Nucleic Acids

SUMMARY A chemical process that enables reversible, covalent RNA modification-highly selective for uridine enabling diverse functional group attachment, facilitating nucleic acid labeling The Unmet Need: Reversible labeling of native RNA in a sequence-independent and membrane-selective manner  … Read More

Low-Input Methylation Sequencing For Profiling Epitranscriptomic Signatures And Early Colorectal Cancer Diagnosis

SUMMARY A method that analyzes cfRNA methylation in plasma to distinguish human RNA from microbiome RNA, accurately detecting colorectal cancer through specific methylation markers even in ultralow input samples. The Unmet Need: Effective biomarkers for early detection of colorectal cancer Current advancements… Read More