Epigenetics

DMN-seq: DEMETER-Assisted 5-Methylcytosine Nicking Sequencing Profiling From Sub-Nanogram Cell-Free DNA

SUMMARY More sensitive methylation profiling from scarce clinical samples is supported by a sequencing method that uses 5mC-specific glycosylase activity to detect methylated sites at single-base resolution and enrich hypomethylated DNA The Unmet Need: Existing methylation methods struggle to profile hypomethylated DNA in scarce clinical samples Bisulfite and related conversion… Read More

Low-Input Methylation Sequencing For Profiling Epitranscriptomic Signatures And Early Colorectal Cancer Diagnosis

SUMMARY A method that analyzes cfRNA methylation in plasma to distinguish human RNA from microbiome RNA, accurately detecting colorectal cancer through specific methylation markers even in ultralow input samples. The Unmet Need: Effective biomarkers for early detection of colorectal cancer Current advancements… Read More